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KMID : 0043320130360050579
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Archives of Pharmacal Research
2013 Volume.36 No. 5 p.579 ~ p.585
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A simultaneous analysis method for Caihu-Dayuan-Yin using reversed-phase high-performance liquid chromatography coupled with pulsed amperometric detection
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Kwon Ha-Jeong
Kim Ji-Hwan
Park Yong-Duk
Hong Seon-Pyo
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Abstract
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A simultaneous analysis method was developed for Caihu-Dayuan-Yin decoction containing Bupleuri Radix, Glycyrrhizae Radix, and Ponciri fructus by reversed-phase (RP) HPLC coupled with pulsed amperometric detection (PAD). The analytes were separated in a RP column with 0.01 % phosphoric acid?acetonitrile gradient elution. The limits of detection (S/N = 3) and of quantification (S/N = 10) ranged 0.1?3.0, and 0.3?10 ng, respectively. The intra- and interday precisions (RSDs) were ≤15.15 % and the average recoveries were 94.6?98.3 %. Our RP-HPLC?PAD method showed 1.7?100 times higher sensitivity than the RP-HPLC?UV method.
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KEYWORD
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Reversed-phased high performance liquid chromatography, Pulsed amperometric detection, Caihu-Dayuan-Yin, Sensitivity
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